Difference between revisions of "Part:BBa K1321326:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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BBa_K1321302 was created by restricting BBa_K1033280 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.
 
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===Source===
 
===Source===

Latest revision as of 15:52, 6 May 2016

pTet01


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4685
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4685
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
    Illegal NotI site found at 1822
    Illegal NotI site found at 4691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4685
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4685
    Illegal XbaI site found at 4700
    Illegal SpeI site found at 946
    Illegal PstI site found at 1829
    Illegal NgoMIV site found at 3004
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References