Difference between revisions of "Part:BBa K1713003"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1713003 short</partinfo>
 
<partinfo>BBa_K1713003 short</partinfo>
  
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Planning
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The hybrid promoter ( Plac/ara-1 ) can be activated by the araC protein in the presence of arabinose and so that GFP produced. And the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).
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<p>Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of  hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:12, 20 January 2016

Plac/ara-1+strong RBS+GFP(+LVA)+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the araC protein in the presence of arabinose and so that GFP produced. And the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 772