Difference between revisions of "Part:BBa S05292"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_S05292 short</partinfo>
 
<partinfo>BBa_S05292 short</partinfo>
  
 
Construction intermediate
 
Construction intermediate
 +
<p>Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of  hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:08, 20 January 2016

Plac/ara-1+strong RBS+LacI(+LVA)

Construction intermediate

Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]