Difference between revisions of "Part:BBa M36533:Design"

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===Source===
 
  
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DNA 2.0 for general plasmid design[https://www.dna20.com/eCommerce/catalog/datasheet/188] including restriction sites, GAL1 inducible promoter, high copy number, and sequence for PHO84 gene [http://www.yeastgenome.org/locus/S000004592/sequence]
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'''Glycerol Freezer Stock'''
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Plasmid: PHO84
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DNA 2.0 GeneID: 232146
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Barcode: 0133029030
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Unique ID: PKPHO8411/18/15
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===Source===
  
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RBS consensus yeast Kozak sequence taken from the Silver Lab, BBa_J63003[https://parts.igem.org/Part:BBa_J63003] was used.
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DNA 2.0 for plasmid backbone [https://www.dna20.com/eCommerce/catalog/datasheet/188] including restriction sites, GAL1 inducible promoter, high copy number ORI, and ampicillin and leucine selectable markers. The sequence for the PHO84 gene was attained from YeastGenome.Org [http://www.yeastgenome.org/locus/S000004592/sequence]
  
 
===References===
 
===References===

Latest revision as of 21:15, 11 December 2015

Arsenate Bioremediation Actuator Plasmid Containing PHO84 Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1438
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Number of repeats were limited to less than 5 for each 10+ base pair sequence



http://i67.tinypic.com/2cegjtt.jpg


Glycerol Freezer Stock

Plasmid: PHO84

DNA 2.0 GeneID: 232146

Barcode: 0133029030

Unique ID: PKPHO8411/18/15

Source

DNA 2.0 for plasmid backbone [1] including restriction sites, GAL1 inducible promoter, high copy number ORI, and ampicillin and leucine selectable markers. The sequence for the PHO84 gene was attained from YeastGenome.Org [http://www.yeastgenome.org/locus/S000004592/sequence]

References