Difference between revisions of "Part:BBa M36533:Design"

(→‎Design Notes)
 
(9 intermediate revisions by the same user not shown)
Line 11: Line 11:
  
  
−
http://imageshack.com/a/img907/2517/DITZFh.png
+
http://i67.tinypic.com/2cegjtt.jpg
 +
 
 +
 
 +
'''Glycerol Freezer Stock'''
 +
 
 +
Plasmid: PHO84
 +
 
 +
DNA 2.0 GeneID: 232146
 +
 
 +
Barcode: 0133029030
 +
 
 +
Unique ID: PKPHO8411/18/15
  
 
===Source===
 
===Source===
  
−
DNA 2.0  
+
DNA 2.0 for plasmid backbone [https://www.dna20.com/eCommerce/catalog/datasheet/188] including restriction sites, GAL1 inducible promoter, high copy number ORI, and ampicillin and leucine selectable markers. The sequence for the PHO84 gene was attained from YeastGenome.Org [http://www.yeastgenome.org/locus/S000004592/sequence]
  
 
===References===
 
===References===

Latest revision as of 21:15, 11 December 2015

Arsenate Bioremediation Actuator Plasmid Containing PHO84 Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1438
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1647
    Illegal PstI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Number of repeats were limited to less than 5 for each 10+ base pair sequence



http://i67.tinypic.com/2cegjtt.jpg


Glycerol Freezer Stock

Plasmid: PHO84

DNA 2.0 GeneID: 232146

Barcode: 0133029030

Unique ID: PKPHO8411/18/15

Source

DNA 2.0 for plasmid backbone [1] including restriction sites, GAL1 inducible promoter, high copy number ORI, and ampicillin and leucine selectable markers. The sequence for the PHO84 gene was attained from YeastGenome.Org [http://www.yeastgenome.org/locus/S000004592/sequence]

References