Difference between revisions of "Part:BBa M36534:Design"
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For self-cleaving 2A peptide sequence, [http://blog.addgene.org/plasmids-101-multicistronic-vectors]
 
For self-cleaving 2A peptide sequence, [http://blog.addgene.org/plasmids-101-multicistronic-vectors]
  
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The sequences for the ACR 1 and 2 genes wer attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]
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The sequences for the ACR 1 and 2 genes were attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]
  
 
===References===
 
===References===

Revision as of 07:49, 8 December 2015

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ACR 2 was ordered first due its increased importance (reducing arsenate to arsenite and conferring arsenite resistance) vs. ACR 1.


http://imageshack.com/a/img907/254/OL6vcf.png

Source

DNA 2.0 for plasmid backbone [1] including restriction sites, GAL1 inducible promoter, high copy number ORI, and kanamycin and histidine selectable markers.

For self-cleaving 2A peptide sequence, [http://blog.addgene.org/plasmids-101-multicistronic-vectors]

The sequences for the ACR 1 and 2 genes were attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]

References