Difference between revisions of "Part:BBa M36534:Design"

Revision as of 07:48, 8 December 2015

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 873
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 873
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 873
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 873
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 873
1000
COMPATIBLE WITH RFC[1000]

Design Notes

ACR 2 was ordered first due its increased importance (reducing arsenate to arsenite and conferring arsenite resistance) vs. ACR 1.

http://imageshack.com/a/img907/254/OL6vcf.png

Source

DNA 2.0 for plasmid backbone [1] including restriction sites, GAL1 inducible promoter, high copy number ORI, and kanamycin and histidine selectable markers.

For self-cleaving 2A peptide sequence, [http://blog.addgene.org/plasmids-101-multicistronic-vectors]

The sequences for the ACR 1 and 2 genes wer attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]

@@ Line 15: / Line 15: @@
 DNA 2.0 for plasmid backbone [https://www.dna20.com/eCommerce/catalog/datasheet/189] including restriction sites, GAL1 inducible promoter, high copy number ORI, and kanamycin and histidine selectable markers.
-For self-cleaving 2A peptide sequence, "INSERT SOURCE"
+For self-cleaving 2A peptide sequence, [http://blog.addgene.org/plasmids-101-multicistronic-vectors]
 The sequences for the ACR 1 and 2 genes wer attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]
 ===References===