Difference between revisions of "Part:BBa M36534:Design"
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===Source=== | ===Source=== | ||
− | DNA 2.0 for general plasmid design[https://www.dna20.com/eCommerce/catalog/datasheet/189] including restriction sites, GAL1 inducible promoter, high copy number, and sequence for ACR 1 and 2 from yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence] | + | DNA 2.0 for general plasmid design[https://www.dna20.com/eCommerce/catalog/datasheet/189] including restriction sites, GAL1 inducible promoter, high copy number ORI, and kanamycin and histidine selectable markers. The sequence for ACR 1 and 2 was attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence] |
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===References=== | ===References=== |
Revision as of 07:04, 8 December 2015
Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 873
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 873
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 873
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 873
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 873
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ACR 2 was ordered first due its increased importance (conferring arsenite resistance) vs. ACR 1.
http://imageshack.com/a/img907/254/OL6vcf.png
Source
DNA 2.0 for general plasmid design[1] including restriction sites, GAL1 inducible promoter, high copy number ORI, and kanamycin and histidine selectable markers. The sequence for ACR 1 and 2 was attained from the yeastgenome website [http://www.yeastgenome.org/locus/S000006403/sequence] [http://www.yeastgenome.org/locus/S000006404/sequence]