Difference between revisions of "Part:BBa M36142:Experience"
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===Applications of BBa_M36142=== | ===Applications of BBa_M36142=== | ||
This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher. | This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher. | ||
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When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made. | When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made. | ||
Revision as of 04:24, 5 December 2015
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Applications of BBa_M36142
This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher.
When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.
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UNIQ7f8ba9c558d99aae-partinfo-00000000-QINU UNIQ7f8ba9c558d99aae-partinfo-00000001-QINU