Difference between revisions of "Part:BBa M36402:Experience"
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Box Label: BioE44 S15 Box 2 | Box Label: BioE44 S15 Box 2 | ||
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===Applications of BBa_M36402=== | ===Applications of BBa_M36402=== | ||
This part has not been used in any other composite parts. | This part has not been used in any other composite parts. | ||
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| + | ===Performance Data of BBa_M36402=== | ||
| + | In order to assay if this plasmid design successfully created the ester methyl salicylate in the presence of salicylic acid we created tubes of transfected E.coli with varying concentration of salicyclic acid and ran these tubes is a GC-MS machine to see if levels of the desired product, methyl salicylate, were detectable. the tubes contained .001mM, .01mM, .1mM, 1.M, and 10mM of salicylic acid. When comparing our plasmid GC-MS readings with prior reading we had gained by testing pure salicylic acid we discovered that this plasmid design did not produce any detectable levels of methyl salicylate and conclusively was an ineffective part. | ||
| + | [[File:Assay 1 Graph_1.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 00:07, 4 December 2015
Contents
Stanford Location
Plasmid Name: WTC_DNA2
DNA 2.0 Gene#: 231900
Organism: E.coli
Device Type: Actuator
Barcode # of Glycerol Stock: 133021775
Box Label: BioE44 S15 Box 2
Applications of BBa_M36402
This part has not been used in any other composite parts.
Performance Data of BBa_M36402
In order to assay if this plasmid design successfully created the ester methyl salicylate in the presence of salicylic acid we created tubes of transfected E.coli with varying concentration of salicyclic acid and ran these tubes is a GC-MS machine to see if levels of the desired product, methyl salicylate, were detectable. the tubes contained .001mM, .01mM, .1mM, 1.M, and 10mM of salicylic acid. When comparing our plasmid GC-MS readings with prior reading we had gained by testing pure salicylic acid we discovered that this plasmid design did not produce any detectable levels of methyl salicylate and conclusively was an ineffective part.
User Reviews
UNIQc64a1f106a495df2-partinfo-00000000-QINU UNIQc64a1f106a495df2-partinfo-00000001-QINU