Difference between revisions of "Part:BBa M36401:Experience"
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[[File:Assay 1 Graph.jpg]]__NOTOC__
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_M36401===
 
 
===Stanford Location===
 
===Stanford Location===
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Plasmid Name:WTC_DNA1
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Plasmid Name: WTC_DNA1
  
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DNA 2.0 Gene#:231899
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DNA 2.0 Gene#: 231899
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Organism:'' E.coli''
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Organism: '' E.coli''
  
 
Device Type: Actuator
 
Device Type: Actuator
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Barcode # of Glycerol Stock: 0133018576
 
Barcode # of Glycerol Stock: 0133018576
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Boz Label: BioE44 S15 Box 2
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Box Label: BioE44 S15 Box 2
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===Applications of BBa_M36401===
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This part has not been used in any other composite parts.
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===Performance Data of BBa_M36401===
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Our first trial showed results that were promising. When using varying amounts of salicylic acid, we were able to observe an increasing amount of methyl salicylate produced for increasing salicylic acid concentrations. We took data for amount of methyl salicylate produced using the GC-MS. We used a logarithmic scale for the amount of salicylic acid added and added .001 mM, .01 mM, .1 mM, 1 mM, 10 mM of salicylic acid. We did not see any production of methyl salicylate at 10 mM which means the concentration of salicylic acid was high enough to kill the E. Coli which was seen as the liquid culture had not growth. We took the methyl salicylate abundance readings from the GC-MS and calculated the mole fractions for the different abundances of methyl salicylate.
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[[File:Asssay 1 Graph.jpg]]
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===User Reviews===
 
===User Reviews===
 
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<I>Username</I>
 
<I>Username</I>
 
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Enter the review inofrmation here.
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Enter the review information here.
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[[File:Assay 1 Graph.jpg]]
 
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Latest revision as of 00:01, 4 December 2015

Stanford Location

Plasmid Name: WTC_DNA1

DNA 2.0 Gene#: 231899

Organism: E.coli

Device Type: Actuator

Barcode # of Glycerol Stock: 0133018576

Box Label: BioE44 S15 Box 2

Applications of BBa_M36401

This part has not been used in any other composite parts.

Performance Data of BBa_M36401

Our first trial showed results that were promising. When using varying amounts of salicylic acid, we were able to observe an increasing amount of methyl salicylate produced for increasing salicylic acid concentrations. We took data for amount of methyl salicylate produced using the GC-MS. We used a logarithmic scale for the amount of salicylic acid added and added .001 mM, .01 mM, .1 mM, 1 mM, 10 mM of salicylic acid. We did not see any production of methyl salicylate at 10 mM which means the concentration of salicylic acid was high enough to kill the E. Coli which was seen as the liquid culture had not growth. We took the methyl salicylate abundance readings from the GC-MS and calculated the mole fractions for the different abundances of methyl salicylate.

Asssay 1 Graph.jpg

User Reviews

UNIQ6654d211b413da8d-partinfo-00000000-QINU UNIQ6654d211b413da8d-partinfo-00000001-QINU