Difference between revisions of "Part:BBa M36401:Design"
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Revision as of 06:15, 25 October 2015
Salicylic Acid to Methyl Salicylate Actuator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 213
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 213
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 213
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 213
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A design considerations we had was the optimization of codons.We condon optimized this particular DNA sequence using IDT's Codon Optimzation Tool by changing its codons to match the most prevalent tRNAs. This way the protein will be more efficiently translated and make more of the desired product. This is the only relevant design change to this part.
Source
This part is derived from SAMT from Antirrhinus majus.
References
Part:BBa_J45001, who created the actual part that we adapted to fit our project.