Difference between revisions of "Part:BBa M36534"

Line 9: Line 9:
 
Part contains (in order):  
 
Part contains (in order):  
 
RBS: consensus yeast Kozak Sequence
 
RBS: consensus yeast Kozak Sequence
 +
 
Spacer sequence
 
Spacer sequence
 +
 
ACR2 gene  
 
ACR2 gene  
 +
 
Stop codon
 
Stop codon
 +
 
Spacer sequence  
 
Spacer sequence  
 +
 
RBS: consensus yeast Kozak Sequence  
 
RBS: consensus yeast Kozak Sequence  
 +
 
Spacer sequence
 
Spacer sequence
 +
 
ACR1 gene
 
ACR1 gene
 +
 
Stop codon
 
Stop codon
  

Revision as of 02:43, 25 October 2015

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.

DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and leucine as the selectable marker


Part contains (in order): RBS: consensus yeast Kozak Sequence

Spacer sequence

ACR2 gene

Stop codon

Spacer sequence

RBS: consensus yeast Kozak Sequence

Spacer sequence

ACR1 gene

Stop codon


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]