Difference between revisions of "Part:BBa M36534"
Line 4: Line 4:
 
This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.
 
This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.
  
−
DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and Uracil as the selectable marker  
+
DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and leucine as the selectable marker  
 +
 
 +
 
 
Part contains (in order):  
 
Part contains (in order):  
 
RBS: consensus yeast Kozak Sequence
 
RBS: consensus yeast Kozak Sequence

Revision as of 02:42, 25 October 2015

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.

DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and leucine as the selectable marker


Part contains (in order): RBS: consensus yeast Kozak Sequence Spacer sequence ACR2 gene Stop codon Spacer sequence RBS: consensus yeast Kozak Sequence Spacer sequence ACR1 gene Stop codon


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]