Difference between revisions of "Part:BBa M36580:Design"
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Sequence before RBS: CAGGATTACA | Sequence before RBS: CAGGATTACA | ||
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Sequence after RBS: TAACCTATCG | Sequence after RBS: TAACCTATCG | ||
Revision as of 23:36, 23 October 2015
LasR/PAI complex sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added preceding spacing to qsc102 promoter palindromic binding sequence (included in qsc102 part), as well as spacing flanking the RBS in order to optimize binding. The added spacing sequences are detailed below.
Sequence before RBS: CAGGATTACA
Sequence after RBS: TAACCTATCG
Source
qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ BBa_0030 strong RBS: partsregistry.org GFP coding sequence: BIOE44 pComet DNA sequence