Difference between revisions of "Part:BBa M36627"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_M36029 short</partinfo>
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<partinfo>BBa_M36627 short</partinfo>
  
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This GFP part is completely based on BBa_K648013, with only 4 silent mutations (single-nucleotide substitutions) introduced for 2 purposes:
+
This GFP part is completely based on BBa_K648013 ([https://parts.igem.org/Part:BBa_K648013]), with only 4 silent mutations (single-nucleotide substitutions) introduced for 2 purposes:
 
<br>
 
<br>
 
1. Removing BbsI, BsmBI, and BsaI restriction sites
 
1. Removing BbsI, BsmBI, and BsaI restriction sites
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_M36029 SequenceAndFeatures</partinfo>
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<partinfo>BBa_M36627 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_M36029 parameters</partinfo>
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<partinfo>BBa_M36627 parameters</partinfo>
 
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Latest revision as of 23:33, 23 October 2015

GFP (modified from BBa_K648013)

This GFP part is completely based on BBa_K648013 ([1]), with only 4 silent mutations (single-nucleotide substitutions) introduced for 2 purposes:
1. Removing BbsI, BsmBI, and BsaI restriction sites
2. Limit number of repeat sequences

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]