Difference between revisions of "Part:BBa M36243:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
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Composite
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Pro EP-B2 DNA sequence and optimized E.Coli his-tag from  "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0
  
 
===References===
 
===References===

Latest revision as of 23:14, 22 October 2015


Pro EP-B2 YebF Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 414
    Illegal BglII site found at 837
    Illegal BglII site found at 1506
    Illegal BamHI site found at 804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1174
    Illegal AgeI site found at 1258
    Illegal AgeI site found at 1597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1925


Design Notes

Testing whether Pro Ep-B2 protein is folded property and then successfully secreted via YebF.

Source

Pro EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0

References