Difference between revisions of "Part:BBa K1671001"
Line 13: Line 13:
 
<p>Let PET-28a-Lipase plasmid be transcribed into BL-21 E. coli (the procedure is the same as that of TOP-10 E. coli), then put on substrate media. Pick E. coli into shake flask for 3 hours. Join IPTG solution (0.1 mmol/L) for abduction for 6 hours. Put into centrifugal machine then supernatant liquid (with lipase) for lipase test. The result shows that the colour has changed besides plasmid with lipase (Figure 11), showing that LipB2 gene is correctly expressed in BL21 E. coli. </p>
 
<p>Let PET-28a-Lipase plasmid be transcribed into BL-21 E. coli (the procedure is the same as that of TOP-10 E. coli), then put on substrate media. Pick E. coli into shake flask for 3 hours. Join IPTG solution (0.1 mmol/L) for abduction for 6 hours. Put into centrifugal machine then supernatant liquid (with lipase) for lipase test. The result shows that the colour has changed besides plasmid with lipase (Figure 11), showing that LipB2 gene is correctly expressed in BL21 E. coli. </p>
  
−
<img scr="http://2015.igem.org/File:Sjclipase.png"/>
+
<img scr="http://2015.igem.org/File:Sjclipase.png" width:500 height:300 />
  
  

Revision as of 18:37, 27 September 2015

Lipase

Lipase, full name triacylglycerol acylhydrolase, is the target gene of this part. It is an is an enzyme that catalyzes the hydrolysis of fats, which is able to solve the oil like pollution on the surface of relics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Let PET-28a-Lipase plasmid be transcribed into BL-21 E. coli (the procedure is the same as that of TOP-10 E. coli), then put on substrate media. Pick E. coli into shake flask for 3 hours. Join IPTG solution (0.1 mmol/L) for abduction for 6 hours. Put into centrifugal machine then supernatant liquid (with lipase) for lipase test. The result shows that the colour has changed besides plasmid with lipase (Figure 11), showing that LipB2 gene is correctly expressed in BL21 E. coli.

<img scr="http://2015.igem.org/File:Sjclipase.png" width:500 height:300 />