Difference between revisions of "Part:BBa K1689014:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1689014 short</partinfo> | <partinfo>BBa_K1689014 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Delta alpha segment of LacZ is fused with dCas9, with a 6xhis tag for protein purification. | |
| Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | E.coli | + | ''E.coli'' |
===References=== | ===References=== | ||
Latest revision as of 16:16, 27 September 2015
dCas9-Clact
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1150
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3429 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Delta alpha segment of LacZ is fused with dCas9, with a 6xhis tag for protein purification.
Source
E.coli