Difference between revisions of "Part:BBa K1689016:Design"
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===Source=== | ===Source=== | ||
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Latest revision as of 16:09, 27 September 2015
dCas9-F[1,2]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1150
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3429 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
Source
E.coli