Difference between revisions of "Part:BBa K1689015:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep in mind when using Gibson Assembly;
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There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep it in mind when using Gibson Assembly;
 
There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.
 
There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.
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−
 
  
 
===Source===
 
===Source===

Latest revision as of 16:08, 27 September 2015

F[1,2]-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep it in mind when using Gibson Assembly; There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.

Source

E.coli

References