Difference between revisions of "Part:BBa K1689020:Design"

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<partinfo>BBa_K1689020 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The coding sequence of this fusion protein is very long. If you want to amplify only part of it (i.e. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
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The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
  
 
===Source===
 
===Source===

Latest revision as of 15:48, 27 September 2015

dCas9-delta omega


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.

Source

E.coli

References