Difference between revisions of "Part:BBa K1741014"

Latest revision as of 08:21, 27 September 2015

sfGFP under proD promoter

An isolated constitutive promoter proD (see Davis, 2010) exhibits higher expression rates than proC. Expression profiles of proC and proD are similar in different media. Their expression levels depend on E. coli growth rate, proving that expression driven by those promoters is constitutive.

Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 201

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 <partinfo>BBa_K1741014 short</partinfo>
-A strong insulated constitutive promoter proD (cf. Davis 2010) is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend  on E.coli growth rate.
+An isolated constitutive promoter proD (see Davis, 2010) exhibits higher expression rates than proC. Expression profiles of proC and proD are similar in different media. Their expression levels depend on ''E. coli'' growth rate, proving that expression driven by those promoters is constitutive.
 ===Results===
@@ Line 21: / Line 21: @@
 https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
-The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
+The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
-We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairpin to stop the translation in 23° C.
+We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.