Difference between revisions of "Part:BBa K1741013"
 
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The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
 
The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
  
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We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC 5'UTR to stop the translation in 23° C.
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We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.
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Latest revision as of 08:13, 27 September 2015

sfGFP under proC promoter with a hairpin in 5'UTR

A hairpin introduced to 5’UTR of proC slightly lowers transcription/translation. We are planning to extend it to use it as a thermometer, as for now we do not see temperature dependence.

UAMpoznanproChairpin.jpg

This is the potential hairpin structure.


Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 232