Difference between revisions of "Part:BBa K1741014"
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https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
 
https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
  
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The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
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The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
  
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We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairpin to stop the translation in 23° C.
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We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.
  
  

Revision as of 08:11, 27 September 2015

sfGFP under proD promoter

A strong insulated constitutive promoter proD (cf. Davis 2010) is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend on E.coli growth rate.

Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

We observed that proD is up to three times stronger than proC. The hairpin concept has to be further elaborated upon and improved so as to stop translation in lower temperatures.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 201