Difference between revisions of "Part:BBa K1741014"

 
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<partinfo>BBa_K1741014 short</partinfo>
 
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A strong insulated constitutive promoter proD is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend  on E. coli growth rate.
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A strong insulated constitutive promoter proD is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend  on E.coli growth rate.
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===Results===
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Legend:
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proC [[https://parts.igem.org/Part:BBa_K1741012  BBa_K1741012]]
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proC with a hairpin in 5'UTR [[https://parts.igem.org/Part:BBa_K1741013  BBa_K1741013]]
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proD [[https://parts.igem.org/Part:BBa_K1741014  BBa_K1741014]]
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https://static.igem.org/mediawiki/parts/3/35/Teamuamprocdiffsugarsgraph.png
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https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png
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https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
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The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
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We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.
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<!-- Add more about the biology of this part here
 
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Revision as of 07:34, 27 September 2015

sfGFP under proD promoter

A strong insulated constitutive promoter proD is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend on E.coli growth rate.

Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 201