Difference between revisions of "Part:BBa K1741004"
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Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein. | Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein. | ||
| + | ===Results=== | ||
| + | Legend: | ||
| + | MelS BBa_K1741004 | ||
| + | MelWT BBa_K1741003 | ||
| + | |||
| + | |||
| + | |||
| + | picture https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png | ||
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Revision as of 04:27, 27 September 2015
sfGFP under melibiose promoter (improved 5'UTR)
Twice as high expression (comparing to BBa_K1741003) has been obtained by 5’UTR editing – removal of potential secondary structure which involved RBS and a better positioning of RBS – 7 nt upstream AUG start codon. The modified 5’UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promoter is still weaker than araBAD or rhaBAD, so can be the promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant protein.
Results
Legend: MelS BBa_K1741004 MelWT BBa_K1741003
picture 
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 207