Difference between revisions of "Part:BBa K1365020:Experience"
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===Utah State 2015 iGEM Team Use=== | ===Utah State 2015 iGEM Team Use=== | ||
| − | <p>The Utah State 2015 iGEM Team paired this fluorescent protein with three ''Lactococcus lactis'' constitutive promoters and tested function in ''Escherichia coli''. It showed activity relative to the strength of the promoters (see Figure 1). | + | <p>The Utah State 2015 iGEM Team paired this fluorescent protein with three ''Lactococcus lactis'' constitutive promoters and tested function in ''Escherichia coli''. It showed activity relative to the strength of the promoters (see Figure 1).</p> |
https://static.igem.org/mediawiki/2015/a/ad/Utah_State_2015_sfGFP%28Bs%29_Fluorescence_Chart_Version3.jpeg | https://static.igem.org/mediawiki/2015/a/ad/Utah_State_2015_sfGFP%28Bs%29_Fluorescence_Chart_Version3.jpeg | ||
<p>Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 485/20 with emissions read at 528/20. cp8 from part BBa_K1820014, cp11 from part BBa_K1820015, and cp44 from part BBa_K1820016.</p> | <p>Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 485/20 with emissions read at 528/20. cp8 from part BBa_K1820014, cp11 from part BBa_K1820015, and cp44 from part BBa_K1820016.</p> | ||
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| + | https://static.igem.org/mediawiki/parts/d/de/Utah_State_2015_Fluorescence_small.jpg | ||
| + | <p>Figure 2. Fluorescence ''E.coli'' bacteria with ''Lactococcus lactis'' promoters and sfGFP(Bs) (left) and mCherry(Lr) (right)</p> | ||
<p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/ </p> | <p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/ </p> | ||
Latest revision as of 01:19, 27 September 2015
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Utah State 2015 iGEM Team Use
The Utah State 2015 iGEM Team paired this fluorescent protein with three Lactococcus lactis constitutive promoters and tested function in Escherichia coli. It showed activity relative to the strength of the promoters (see Figure 1).
Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 485/20 with emissions read at 528/20. cp8 from part BBa_K1820014, cp11 from part BBa_K1820015, and cp44 from part BBa_K1820016.
Figure 2. Fluorescence E.coli bacteria with Lactococcus lactis promoters and sfGFP(Bs) (left) and mCherry(Lr) (right)
Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/
Applications of BBa_K1365020
This part was used in the composite part BBa_K1365555 for characterization.
The graph shown above helps us to know distribution of the fluorescence in the cells of E.coli with different CP promotor fused to superfoldedGFP-terminator-RBS collection (CP1, CP11, CP29, CP30, CP41, CP44) and J23101 as reference. We used this method to characterize the CP promoter collection that was BioBricked by the Uppsala 2013 team.
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