Difference between revisions of "Part:BBa K1820002"

 
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<partinfo>BBa_K1820002 short</partinfo>
 
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<p>A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31.</p>
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<p> This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes. </p>
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<p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p>
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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<partinfo>BBa_K1820002 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K1820002 parameters</partinfo>
 
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===References===
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<p>Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.</p>

Latest revision as of 16:13, 26 September 2015

P335-Φ31phage genetic switch-divergent promoter

A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31.

Usage and Biology

This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.