Difference between revisions of "Part:BBa K1820000"
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| − | <p> | + | <p> Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p> |
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| + | <p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p> | ||
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Latest revision as of 16:12, 26 September 2015
P335-Φ31 inducible middle promoter
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.
Usage and Biology
Transcription from this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.
This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
References
Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.