Difference between revisions of "Part:BBa K1645999:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
===Applications of BBa_K1645999=== | ===Applications of BBa_K1645999=== | ||
| + | We used Imaging Flow Cytometry to characterise this part, as it allowed us to visualise the expression of both red and green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB overnight. The cells were fixed and diluted 10 times in 10% formalin buffer before use in Imaging FLow Cytometry, where they were excited by a laser optimised for reading at 488nm. In order to control for signal leakage between channels, BBa_I20260 and BBa_J04450 were used as single colour controls. This intensity plot of both colours was created. | ||
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| + | The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. [[Link title]] | ||
===User Reviews=== | ===User Reviews=== | ||
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<!-- Template for a user review | <!-- Template for a user review | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
| − | |- | + | |-[http://www.example.com link title] |
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K1645999 AddReview number</partinfo> | <partinfo>BBa_K1645999 AddReview number</partinfo> | ||
Latest revision as of 12:53, 26 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1645999
We used Imaging Flow Cytometry to characterise this part, as it allowed us to visualise the expression of both red and green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB overnight. The cells were fixed and diluted 10 times in 10% formalin buffer before use in Imaging FLow Cytometry, where they were excited by a laser optimised for reading at 488nm. In order to control for signal leakage between channels, BBa_I20260 and BBa_J04450 were used as single colour controls. This intensity plot of both colours was created.
The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. Link title
User Reviews
UNIQd159017c34076341-partinfo-00000000-QINU UNIQd159017c34076341-partinfo-00000001-QINU