Difference between revisions of "Part:BBa K1613012:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream. | |
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===Source=== | ===Source=== |
Revision as of 03:10, 26 September 2015
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 642
Illegal AgeI site found at 754 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream.
Source
Promoter, RBS, Terminator, and RFP are came from IGEM plate.