Difference between revisions of "Part:BBa K1618046"

Latest revision as of 02:31, 26 September 2015

THL.BHBD.CRO.BCD.EFb.EFa.BUK.PTB

K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate ¹. At the last moment, we realised that there was an EcoRI site on the gene sequence, we're working to fix it as soon as possible.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 229
Illegal suffix found in sequence at 7178
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 7222
Illegal SpeI site found at 8122
Illegal PstI site found at 8136
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal NheI site found at 1167
Illegal SpeI site found at 7179
Illegal SpeI site found at 8122
Illegal PstI site found at 7193
Illegal PstI site found at 8136
Illegal NotI site found at 235
Illegal NotI site found at 3841
Illegal NotI site found at 7186
Illegal NotI site found at 7213
Illegal NotI site found at 8129
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal BglII site found at 1415
Illegal BglII site found at 6388
Illegal BglII site found at 6628
Illegal BglII site found at 6769
Illegal BglII site found at 7546
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 229
Illegal suffix found in sequence at 7179
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 7222
Illegal SpeI site found at 8122
Illegal PstI site found at 8136
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 244
Illegal XbaI site found at 7222
Illegal SpeI site found at 7179
Illegal SpeI site found at 8122
Illegal PstI site found at 7193
Illegal PstI site found at 8136
Illegal AgeI site found at 1338
Illegal AgeI site found at 3354
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 7372

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1618046 short</partinfo>
-K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate.
+K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate <sup>1</sup>. At the last moment, we realised that there was an EcoRI site on the gene sequence, we're working to fix it as soon as possible.
+. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, <i>Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene</i> , Environmental Microbiology, 12(2) p. 304–14.
 [[File:NRP-UEA-Norwich-Flavs-Thing.png]]