Difference between revisions of "Part:BBa K1618013"

Latest revision as of 02:25, 26 September 2015

Plac-PTB

K1618013 contains the coding sequence for the enzyme phosphotransbutrylase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Phosphotransbutrylase catalyzes the Conversion of Butyryl to/from Butyryl-CoA ¹.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 546
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 372

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1618013 short</partinfo>
-Phosphotransbutrylase with IPTG inducible Promoter
+K1618013 contains the coding sequence for the enzyme phosphotransbutrylase of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Phosphotransbutrylase catalyzes the Conversion of Butyryl to/from Butyryl-CoA <sup>1</sup>.
+. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, <i>Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene</i> , Environmental Microbiology, 12(2) p. 304–14.
+[[File:NRP-UEA-Norwich-Flavs-Thing.png]]
+<i>Figure 1: The butyrate biosynthetic pathway. </i>
 <!-- Add more about the biology of this part here