Difference between revisions of "Part:BBa K1680009"

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[[Image:PSUC-conc.png|400px]]
 
[[Image:PSUC-conc.png|400px]]
 +
 
RLU measurement of the NanoLuc under pSUC2 promoter [[BBa_K950003]] control, normalized to OD600.  
 
RLU measurement of the NanoLuc under pSUC2 promoter [[BBa_K950003]] control, normalized to OD600.  
  
 
[[Image:Team Tuebingen mpr 6h.jpg|400px]]
 
[[Image:Team Tuebingen mpr 6h.jpg|400px]]
 +
 
RLU measurement of the NanoLuc under pFET3 promoter [[BBa_K950000]] control, normalized to OD600.  
 
RLU measurement of the NanoLuc under pFET3 promoter [[BBa_K950000]] control, normalized to OD600.  
  

Revision as of 00:25, 26 September 2015

NanoLuc Luciferase

This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.


PSUC-conc.png

RLU measurement of the NanoLuc under pSUC2 promoter BBa_K950003 control, normalized to OD600.

Team Tuebingen mpr 6h.jpg

RLU measurement of the NanoLuc under pFET3 promoter BBa_K950000 control, normalized to OD600.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]