Difference between revisions of "Part:BBa K1680009"
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This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings. | This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings. | ||
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+ | [[Image:PSUC-conc.png|400px]] | ||
+ | RLU measurement of the NanoLuc under pSUC2 promoter [[BBa_K950003]] control, normalized to OD600. | ||
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+ | [[Image:Team Tuebingen mpr 6h.jpg|400px]] | ||
+ | RLU measurement of the NanoLuc under pFET3 promoter [[BBa_K950000]] control, normalized to OD600. | ||
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Revision as of 00:25, 26 September 2015
NanoLuc Luciferase
This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.
RLU measurement of the NanoLuc under pSUC2 promoter BBa_K950003 control, normalized to OD600.
RLU measurement of the NanoLuc under pFET3 promoter BBa_K950000 control, normalized to OD600.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]