Difference between revisions of "Part:BBa K1613005"

Revision as of 21:13, 25 September 2015

A composite part that can detect some toxins.

Introduction

cyp1a2 can oxidize the substances that it can metabolism.

Cytochrome P450 1A2 is a member of the cytochrome P450 mixed-function oxidase system. And it is involved in the metabolism of xenobiotics in the body. Function The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1A2 is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include aflatoxin B1, and acetaminophen.

Substrates

many antidepressants amitriptyline clomipramine some atypical antipsychotics clozapine olanzapine ropivacaine theophylline zolmitriptan melatonin tamoxifen erlotinib cyclobenzaprine estradiol fluvoxaminemexiletine naproxen ondansetron phenacetin paracetamol propranolol riluzole tacrine tizanidine verapamil warfarin

Design

When aflatoxin B1 goes into the e.coli, CYP1A2 will oxidize the Aflatoxin B1, and then the aflatoxin B1 will turn into Aflatoxin oxidation AFBO. The Aflatoxin oxidation will cause DNA damage, making DNA become single-strand DNA(ssDNA), which can activate protein RecA. E.coli has a sos response system, and there is a repressor LexA which inhibits the next reaction, so the red fluorescent gene won’t express. But when the activated protein RecA comes and hydrolysis the repressor LexA, the next reaction can work, so the fluorescent gene will express. Therefore, we can know that there is Aflatoxin B1 in the oil we test. Sources

Sequence and Features