Difference between revisions of "Part:BBa K1676100"
(One intermediate revision by the same user not shown) | |||
Line 2: | Line 2: | ||
<partinfo>BBa_K1676100 short</partinfo> | <partinfo>BBa_K1676100 short</partinfo> | ||
− | + | ||
+ | This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control. | ||
+ | Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1. | ||
Mutant library constructed via site directed mutagenesis: | Mutant library constructed via site directed mutagenesis: | ||
Line 8: | Line 10: | ||
https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png | https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png | ||
− | Shown below are the mutants of the RBS with the same reporter. 1AT denote substitution of A to T. | + | Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T. |
https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg | https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg |
Latest revision as of 18:36, 25 September 2015
GFP Reporter with pLac as promoter
This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control.
Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.
Mutant library constructed via site directed mutagenesis:
Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725