Difference between revisions of "Part:BBa K1676100"

Latest revision as of 18:36, 25 September 2015

GFP Reporter with pLac as promoter

This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control. Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.

Mutant library constructed via site directed mutagenesis:

Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 725

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1676100 short</partinfo>
-For GFP measurement as control. Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.
+This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control.
+Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.
 Mutant library constructed via site directed mutagenesis:
@@ Line 8: / Line 10: @@
 https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png
-Shown below are the mutants of the RBS with the same reporter. 1AT denote substitution of A to T.
+Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T.
 https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg
+https://static.igem.org/mediawiki/2015/9/90/Sumary.png
 <!-- Add more about the biology of this part here