Difference between revisions of "Part:BBa K1676100"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1676100 short</partinfo>
 
<partinfo>BBa_K1676100 short</partinfo>
  
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For GFP measurement as control
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This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control.
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Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.
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Mutant library constructed via site directed mutagenesis:
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https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png
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Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T.
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https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg
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https://static.igem.org/mediawiki/2015/9/90/Sumary.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:36, 25 September 2015

GFP Reporter with pLac as promoter


This GFP reporter contains the wild type RBS, B0034 as was used in the measurement of our mutant library as a control. Our team, NTU igem has created a single basepair mutant library for the RBS, BBa_B0034. We have characterised this mutant in Shewanella oneidensis MR1.

Mutant library constructed via site directed mutagenesis:

MUTATIONS.png

Shown below are the mutants of the RBS with the same reporter which are spotted when OD600 = 0.4. 1AT denote substitution of A to T.

Rbs-spots.jpeg

Sumary.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725