Difference between revisions of "Part:BBa K1859020:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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<html>
 
 
===Applications of BBa_K1859020===
 
===Applications of BBa_K1859020===
  
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<p>
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<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
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</a> .
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</p>
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<p>
 
We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 
We inserted this generator into <i>E. coli</i> K-12 JM109.<br>
 
We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
 
We cultivated this bacteria and performed SDS-PAGE without boiling.<br>
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Lane E shows this sample.<br>
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Lane E shows this sample.<br><br>
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</p>
  
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<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="111" height="160" vspace="" hspace="" align="left"></img>
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<img src="https://static.igem.org/mediawiki/2015/0/04/Gifu-sds-before.jpeg" width="" height="240" vspace="10" hspace="50" align=""></img>
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<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="146" height="160" vspace="10" hspace="50" align="right"></img>
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<img src="https://static.igem.org/mediawiki/2015/a/ab/Gifu-sds-dye.jpg" width="" height="240" vspace="10" hspace="50" align=""></img>
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<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="240" vspace="10" hspace="50" align=""></img>
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<br>left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
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<br><br>
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<p>
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There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.</p>
  
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<b>Fluorescence of protein before dying by CBB</b>     
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Fig.5 after dying by CBB<br><br></b></p>
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<img src="https://static.igem.org/mediawiki/2015/6/60/Gifu-sds-page-combine.png" width="" height="160" vspace="10" hspace="50" align="left"></img>
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<b>Fig.6 Overlapping Fig.4 and Fig.5 </b><br>
 
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&nbsp;&nbsp;There was not fluorescence at the place of long chain protein. We found that long chain protein didn’t have a function.
 
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===User Reviews===
 
===User Reviews===

Latest revision as of 05:10, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859020

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane E shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.


User Reviews

UNIQ19b68d67242b3d0c-partinfo-00000001-QINU UNIQ19b68d67242b3d0c-partinfo-00000002-QINU