Difference between revisions of "Part:BBa K1602049"
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This part is half of a two-part riboregulator-system for <i>E.coli</i> for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the RBS enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are <html><a href="/Part:BBa_K1602050">RRlocked</a></html> and <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html>.
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<figcaption><b>Figure 1:</b> Interaction of taRNA and crRNA leads to gene expression.</figcaption>
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        <h1>RR_KEY</h1>
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                <b>RR_KEY</b> is a trans aktivating RNA which can be put behind every possible promotor.
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The sequence of the taRNA is based on a existing riboregulator sequence pair published by Isaacs et al[1]. The original sequence contained an EcoRI restriction site which was removed by basepair-exchange. We used the <html><a href="http://rsdnerf.com/">Riboswitch Designer</a></html> to find out which basepair-exchange is best suited to remove the unwanted EcoRI restriction site while not affecting the folding- and interaction-capabilities of the sequence.
  
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===Functional Parameters===
 
===Functional Parameters===
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===References===
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1. Isaacs, F.J., et al., Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol, 2004. 22(7): p. 841-7.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602049 parameters</partinfo>
 
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Revision as of 03:19, 25 September 2015

RRkey

This part is half of a two-part riboregulator-system for E.coli for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the RBS enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are RRlocked and RRlocked_site.

Figure 1: Interaction of taRNA and crRNA leads to gene expression.

The sequence of the taRNA is based on a existing riboregulator sequence pair published by Isaacs et al[1]. The original sequence contained an EcoRI restriction site which was removed by basepair-exchange. We used the Riboswitch Designer to find out which basepair-exchange is best suited to remove the unwanted EcoRI restriction site while not affecting the folding- and interaction-capabilities of the sequence.

Functional Parameters

References

1. Isaacs, F.J., et al., Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol, 2004. 22(7): p. 841-7.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]