Difference between revisions of "Part:BBa K1717171"

Revision as of 01:29, 25 September 2015

Protein coding sequence for KeratinaseA

Keratinases are proteolytic enzymes which break the disulphide bonds in various keratin substrates. KERA is a basic part comprised from a synthesized KERA protein coding sequence, optimized for expression in E. coli and most active in degrading keratin in feathers. This gene, when expressed in cells, can be tested for activity through looking for zones of clearing on skim milk agar plates where cells are growing, as well as by looking at degradation of keratin-containing substrates (feathers, hair, chemically synthesized keratin, etc.) when grown with cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 130
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 391
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 761
Illegal SapI site found at 1064

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1717171 short</partinfo>
-Keratinases are proteolytic enzymes which break the disulphide bonds in various keratin substrates. KERA is a basic part comprised from a synthesized KERA protein coding sequence, optimized for expression in E. coli and most active in degrading keratin in hair.
+Keratinases are proteolytic enzymes which break the disulphide bonds in various keratin substrates. KERA is a basic part comprised from a synthesized KERA protein coding sequence, optimized for expression in E. coli and most active in degrading keratin in feathers.
-This gene, when expressed in cells, can be tested for activity through looking for zones of clearing on skim milk agar plates where cells are growing, as well as by looking at degradation of keratin-containing substrates (hair, feathers, chemically synthesized keratin, etc.) when grown with cells.
+This gene, when expressed in cells, can be tested for activity through looking for zones of clearing on skim milk agar plates where cells are growing, as well as by looking at degradation of keratin-containing substrates (feathers, hair, chemically synthesized keratin, etc.) when grown with cells.
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