Difference between revisions of "Part:BBa K1602050"
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        <h1>RRlocked</h1>
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                <b>RRlocked</b> is a RBS masked lock, which is expressed by a constitutive promotor (BBa_J23100). Only if the Key3 (BBa_K1602051) is present, the toxic peptid hokD (BBa_k1497008) is expressed.
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This part is half of a two-part killswitch-system utillizing a riboregulator for posttransciptional regulation of hokD. It consists of the fused sequences of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), a cis-repressing sequence, hokD (<html><a href="/Part:BBa_K1497008">BBa_K1497008</a></html>) and a terminator (<html><a href="/Part:BBa_B0015">BBa_B0015</a></html>). When transcribed the cis-repressing sequence forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence.
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===Functional Parameters===
 
===Functional Parameters===
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===References===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1602050 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1602050 parameters</partinfo>
 
<partinfo>BBa_K1602050 parameters</partinfo>
 
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Revision as of 22:57, 24 September 2015

RRlocked

This part is half of a two-part killswitch-system utillizing a riboregulator for posttransciptional regulation of hokD. It consists of the fused sequences of a constitutive promoter (BBa_J23100), a cis-repressing sequence, hokD (BBa_K1497008) and a terminator (BBa_B0015). When transcribed the cis-repressing sequence forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]