Difference between revisions of "Part:BBa K1859026"
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Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. | Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. | ||
| − | However, [BBa_K1859015] and [BBa_K1859016] were cloned the sequence extra in addition to the ribozyme | + | However, [BBa_K1859015] and [BBa_K1859016] were cloned the sequence extra in addition to the ribozyme. The secondary structure as the mRNA is shown below.<br> |
| − | . | + | |
</p> | </p> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2015/b/b9/Gifu_outside_1.png" width="380" height="" vspace="25" hspace="" align=""></img></center> | ||
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<p> | <p> | ||
Revision as of 20:26, 24 September 2015
mRNA circularize efficiently generator (outside complementarity)
We designed this generator [BBa_K1859026] to improve circular efficiency of mRNA including His-RFP sequence.
First, we improved Circular Parts of iGEM2014,the 5´side of the intron
[BBa_K1332003]
and the 3´side of the intron
[BBa_K1332005]
, and made
[BBa_K1859015]
and
[BBa_K1859016]
.
Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage.
However, [BBa_K1859015] and [BBa_K1859016] were cloned the sequence extra in addition to the ribozyme. The secondary structure as the mRNA is shown below.
The generator is comprised of Circular parts improved to become complementary ( [BBa_K1859015] and [BBa_K1859016] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
The RNA made by this generator [BBa_K1859026] was designed to bind outside of circular mRNA complementary.
We found that circular efficiency of [BBa_K1859026] was the best, when we compared with the amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_K1859024] , [BBa_K1859025] ,[BBa_K1859026].