Difference between revisions of "Part:BBa K1692027"

 
 
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<partinfo>BBa_K1692027 short</partinfo>
 
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Cellulose binding domain adapted from imperial 2014 iGEM team. Last part of our composite part BBa_BBa_K1692028
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<h2> Overview </h2>
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We used the cellulose binding domain cellulosomal-scaffolding protein A (cipA) of <i> Clostridium thermocellum </i> for construction of our cipA-mSA construct ([https://parts.igem.org/Part:BBa_K1692035 BBa_K1692035]) and our spore coat construct ([https://parts.igem.org/Part:BBa_K1692028 BBa_K1692028]). The cipA used is modified to get rid of the illegal ecoRI site found in the 2014 Imperial College IGEM team construct.  
  
 
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Latest revision as of 09:48, 24 September 2015

CIPA-MSA

Overview

We used the cellulose binding domain cellulosomal-scaffolding protein A (cipA) of Clostridium thermocellum for construction of our cipA-mSA construct (BBa_K1692035) and our spore coat construct (BBa_K1692028). The cipA used is modified to get rid of the illegal ecoRI site found in the 2014 Imperial College IGEM team construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]