Difference between revisions of "Part:BBa K1618023"

Latest revision as of 01:21, 23 September 2015

GlgB-Rv3030 with IPTG-inducible promoter

K1618023 contains the coding sequences for GlgB from E. coli and Rv3030 from Mycobacterium tuberculosis (strain ATCC 25618). GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3030 is a putative S-adenosylmethionine-dependent methyltransferase and/or acyltransferase. This part will potentially make branched, acylated glycogen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 283
Illegal BamHI site found at 1491
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 917

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 <partinfo>BBa_K1618023 short</partinfo>
-Rv3030 is a gene which encodes for a putative acyltransferase which will hopefully acylate glycogen chains. This enzyme is encoded with GlgB which codes for a branching enzyme which will cut alpha-1,4 links and re-anneal the strand to the main glycogen compound through an alpha-1,6 linkage, therefore creating a branch. These enzymes are promoted by an IPTG-inducible promoter and are encoded within the pSB1C3 standard vector. The bacteria therefore shows chloramphenicol resistance.
+K1618023 contains the coding sequences for GlgB from <i>E. coli</i> and Rv3030 from <i>Mycobacterium tuberculosis</i> (strain ATCC 25618). GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3030 is a putative S-adenosylmethionine-dependent methyltransferase and/or acyltransferase. This part will potentially make branched, acylated glycogen.
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