Difference between revisions of "Part:BBa K1616005:Experience"
(→‎Applications of BBa_K1616005)
Line 5: Line 5:
 
===Applications of BBa_K1616005===
 
===Applications of BBa_K1616005===
  
−
<h2><font style="color:#b22222"> Notebook </font></h2>
+
<h2> Notebook </h2>
  
 +
<h3><font style="color:#b22222">Ligation of Gblocks Holin-Endolysin into pDawn </font></h3>
  
 +
<table id="Mixligation">
 +
 +
<tbody><tr>
 +
<th>
 +
Tube
 +
</th>
 +
<th>
 +
H/E
 +
</th>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<b>Water</b>
 +
</td>
 +
 +
<td>
 +
4,6 µL
 +
</td>
 +
 +
</tr><tr>
 +
<td>
 +
<b>T4 ligase Buffer</b>
 +
</td>
 +
 +
 +
<td>
 +
1 µL
 +
</td>
 +
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
<b>pDawn</b>
 +
</td>
 +
 +
<td>
 +
1,4 µL
 +
</td>
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
<b>Plasmid</b>
 +
</td>
 +
 +
<td>
 +
2,5 µL
 +
</td>
 +
 +
</tr>
 +
<tr>
 +
<td>
 +
T4 ligase
 +
</td>
 +
 +
<td>
 +
0,5 µL
 +
</td>
 +
 +
</tr>
 +
</tbody></table>
  
−
<h2><font style="color:#b22222"> Results </font></h2>
+
<h2>Results </h2>
  
 
<center>[[Image:Graph-HE.png|center|600px|]] </center>
 
<center>[[Image:Graph-HE.png|center|600px|]] </center>

Revision as of 23:56, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1616005

Notebook

Ligation of Gblocks Holin-Endolysin into pDawn

<tbody> </tbody>

Tube

H/E

Water

4,6 µL

T4 ligase Buffer

1 µL

pDawn

1,4 µL

Plasmid

2,5 µL

T4 ligase

0,5 µL

Results

Graph-HE.png


Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.

In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.

User Reviews

UNIQ5df3095df9bb8414-partinfo-00000000-QINU UNIQ5df3095df9bb8414-partinfo-00000001-QINU