Difference between revisions of "Part:BBa K1692024"

Revision as of 21:20, 22 September 2015

Autolysis

Note: Please click here if you're looking for the msA-cipA construct (BBa_K1692027)

We create an autolysis system based on the concept of quorum sensing. The idea of quorum sensing is that Gram-negative bacteria releases a special molecule known as [N-]acyl-homoserine lactones (AHL) that can bind to the luxR proteins. The complex formed from using 2 AHL molecules with 2 luxR proteins can then bind the luxpR promoter and activate any downstream gene [1]. Our construct combines the part from 2010 Tokyo NoKoGen (BBa_K317039) and our ptet-luxl generator construct (BBa_K1692023). The specific AHL molecule used is 3OC6HSL, which is found in V. fischer . Our goal is to be create an automatic means of self-lysis once the cells reach a certain cell density.

We successfully sequenced the construct and did some preliminary testing with the lysis device. The autolysis system can be used in many applications and one such application is combining autolysis with our designed P(3HB) part (BBa_K1692021) as a means of releasing plastic into the media without having to do extraction process.

References

[1]. Waters, Christopher, and Bassler, Bonnie. "Quorum Sensing:Cell-to-Cell Communication in Bacteria." Annu. Rev. Cell Dev. Biol. 2005 (21): 319–46. Print.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 875
Illegal NheI site found at 898
Illegal NheI site found at 3268
Illegal NheI site found at 3291
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 717
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2873
Illegal AgeI site found at 2943
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1853
Illegal SapI site found at 3524

@@ Line 4: / Line 4: @@
 <b> Note: </b> Please click here if you're looking for the msA-cipA construct ([https://parts.igem.org/Part:BBa_K1692027 BBa_K1692027]) <br><br>
-We create an autolysis system based on the concept of quorum sensing. The idea of quorum sensing is that Gram-negative bacteria releases a  special molecule known as [N-]acyl-homoserine lactones (AHL) that can bind to the luxR proteins. The complex formed from using 2 AHL molecules with 2 luxR proteins can then bind the luxpR promoter and activate any downstream gene [1]. Our construct combines the part from 2010 Tokyo NoKoGen ([https://parts.igem.org/Part:BBa_K317039 BBa_K317039]) and our ptet-luxl generator construct ([https://parts.igem.org/Part:BBa_K1692023 BBa_K1692023]). The specific AHL molecule used is 3OC6HSL, which is found in <i> V. fischer <i>. Our goal is to be create an automatic means of self-lysis once the cells reach a certain cell density. <br>
+We create an autolysis system based on the concept of quorum sensing. The idea of quorum sensing is that Gram-negative bacteria releases a  special molecule known as [N-]acyl-homoserine lactones (AHL) that can bind to the luxR proteins. The complex formed from using 2 AHL molecules with 2 luxR proteins can then bind the luxpR promoter and activate any downstream gene [1]. Our construct combines the part from 2010 Tokyo NoKoGen ([https://parts.igem.org/Part:BBa_K317039 BBa_K317039]) and our ptet-luxl generator construct ([https://parts.igem.org/Part:BBa_K1692023 BBa_K1692023]). The specific AHL molecule used is 3OC6HSL, which is found in <i> V. fischer </i>. Our goal is to be create an automatic means of self-lysis once the cells reach a certain cell density. <br>
 We successfully sequenced the construct and did some preliminary testing with the lysis device. The autolysis system can be used in many applications and one such application is combining autolysis with our designed P(3HB) part ([https://parts.igem.org/Part:BBa_K1692021 BBa_K1692021]) as a means of releasing plastic into the media without having to do extraction process.