Difference between revisions of "Part:BBa K1618046"

 
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<partinfo>BBa_K1618046 short</partinfo>
 
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K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate.
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[[File:NRP-UEA-Norwich-Flavs-Thing.png]]
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<i>Figure 1: The butyrate biosynthetic pathway. </i>
  
 
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<!-- Add more about the biology of this part here

Revision as of 17:23, 22 September 2015

THL.BHBD.CRO.BCD.EFb.EFa.BUK.PTB

K1618046 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β, Electron Transfer Protein α, Butyrate Kinase and Phosphotransbutrylase from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into butyrate.

NRP-UEA-Norwich-Flavs-Thing.png

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 7178
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 7207
    Illegal XbaI site found at 7222
    Illegal SpeI site found at 8122
    Illegal PstI site found at 8136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 7207
    Illegal NheI site found at 1167
    Illegal SpeI site found at 7179
    Illegal SpeI site found at 8122
    Illegal PstI site found at 7193
    Illegal PstI site found at 8136
    Illegal NotI site found at 235
    Illegal NotI site found at 3841
    Illegal NotI site found at 7186
    Illegal NotI site found at 7213
    Illegal NotI site found at 8129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 7207
    Illegal BglII site found at 1415
    Illegal BglII site found at 6388
    Illegal BglII site found at 6628
    Illegal BglII site found at 6769
    Illegal BglII site found at 7546
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal suffix found in sequence at 7179
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 7207
    Illegal XbaI site found at 7222
    Illegal SpeI site found at 8122
    Illegal PstI site found at 8136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 3159
    Illegal EcoRI site found at 7207
    Illegal XbaI site found at 244
    Illegal XbaI site found at 7222
    Illegal SpeI site found at 7179
    Illegal SpeI site found at 8122
    Illegal PstI site found at 7193
    Illegal PstI site found at 8136
    Illegal AgeI site found at 1338
    Illegal AgeI site found at 3354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7372